Translational_Unit
XimE

Part:BBa_K4179018:Design

Designed by: Amit Nelkin   Group: iGEM22_Technion-Israel   (2022-10-06)


SnoaL-like cyclase - XimE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 160
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 160
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of this part was taken from the work of Bu et al.


Source

Streptomyces xiamenensis strain 318

References

Bu, X. L., He, B. B., Weng, J. Y., Jiang, C. C., Zhao, Y. L., Li, S. M., ... & Xu, M. J. (2020). Constructing microbial hosts for the production of benzoheterocyclic derivatives. ACS Synthetic Biology, 9(9), 2282-2290.